- Description
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Y3 Ampicillin (D(-)-a-Aminobenzylpenicillin,
C16H18N3NaO4S, M.W.= 371,39)
is a half-synthetic penicillin (b-lactam antibiotic),
known to interfere with the peptidoglycan cross-linking in grown bacteria
and to inhibit cell wall biosynthesis. Acts at pH 5.5-6.0. Stock solution
is prepared at conc. of 50 mg/ml in water; the working concentration is
20-50 µg/ml.
Y4 Kanamycin A (Kanamycin sulfate, C18H36N4O11
x H2SO4, M.W.=582,58) binds to 70S ribosomal subunit,
inhibits translocation and elicits miscoding. Stock solution is prepared
at conc. of 10 mg/ml in water; the working concentration is 10-50 µg/ml.
The solution is stable in pH range 6-8 at RT for 6-8 month (loss of activity
approx. 1-2%); autoclaving at 120°C for 1 hour results in 5% loss
of activity.
Y5 Tetracycline hydrochloride (C22H24N2O8xHCl,
M.W.=480,9) inhibits protein synthesis (elongation) by preventing binding
of aminoacyl-tRNA to ribosome. Do not use medium with magnesium for selection
of bacteria resistant to Tc because Mg2+ ions are antagonists
of tetracycline. Stock solution is prepared at conc. of 10 mg/ml in 70%
ethanol or in water; the working concentration is 10-50 µg/ml. Use
freshly prepared stock solution; discard after 4 weeks of storage.
Y6 G418 (Geneticin, C20H40N4O10
x H2SO4, M.W.=692,70) is an aminoglycosidic antibiotic
toxic to both pro- and eukaryotic cells. It acts by interfering with protein
synthesis and is used for selection and maintenance of eucaryotic cells
stably transfected with neomycin resistance gene. Stock solution is prepared
at conc. of 40 mg/ml in PBS or water. The working solutions are 400 µg/ml
for selection and 200 µg/ml for maintenance.
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