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One unit of the enzyme will incorporate 10 nmol of dNTPs into an acid-insoluble form in 30 min at 72°C under standard assay conditions using a DNA template. Specific activity is 200.000U/mg protein. |
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B1 (x10, with Mg2+ ): 670 mM Tris-HCl, pH 8.8, 166 mM (NH4)2SO4, 15 mM MgCl2, 0.1% Tween 20. | ||||
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Amplification conditions |
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total |
30 µl |
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Hot-start Steps |
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Number of cycles |
30 cycles |
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Quality control |
activity, SDS-PAGE purity, absence of endo- and exonucleases, specific performance tests. |
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References |
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T16.5 |
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Pack size |
1000 U |
2500 U |
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Copyright © 2002 M.B. Enzymes GmbH