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Abbrevations: R=A or G; Y=C or T; W=A or T; D=A,G or T; N=G,A,T or C. * = pronounced "star" activity a = blocked by dam methylation; c = blocked by dcm methylation; CG = blocked at sites m5CG or m5CNG; (CG) = cleavage blocked only at sites with overlapping CG methylation; iso = isoschizomers (recognize and cleave identical sites); neo = neoschizomers (enzymes having different cleavage specificity). |
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Cleavage site |
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| 1588 Aat II*CG | - Aat II*CG | GACGT/C | 37 A | 40,00 |
| 2113 Vha464 I | iso Afl II | C/TTAAG | 37 M | 25,00 |
| 61 Alu | - Alu I | AG/CT | 37 A | 75,00 |
| 84 Apa Ic | - Apa Ic | GGGCC/C | 37 A | 10,00 |
| 2683 PspOM I | neo Apa Ic | G/GGCCC | 37 A | 25,00 |
| 2116 Vne I | iso ApaL I(CG) | G/TGCAC | 37 H | 25,00 |
| 301 Bpu14 I | iso Asu II | TT/CGAA | 37 M | 25,00 |
| 286 Bme18 I | iso Ava IIc | G/GWCC | 37 H | 25,00 |
| 185 BamH I | - BamH I | G/GATCC | 37 ABM | 2,50 |
| 1182 Ksp22 Ia | iso Bcl Ia | T/GATCA | 37 M | 25,00 |
| 260 BgI I(CG) | - Bgl I(CG) | GCCNNNN/NGGC | 37 H | 25,00 |
| 261 BgI II | - Bgl II | A/GATCT | 37 ABMH | 25,00 |
| 2681 PspE I | iso BstE II | G/GTNACC | 37 L | 25,00 |
| 2745 Bst2U I | iso BstN I | CC/WGG | 60 M | 25,00 |
| 716 Cla Ia CG | - Cla Ia CG | AT/CGAT | 37 ABMH | 50,00 |
| 2671 Bsa29 Ia | iso Cla Ia CG | AT/CGAT | 37 M | 25,00 |
| 778 Dra I | - Dra I | TTT/AAA | 37 AM | 50,00 |
| 993 EcoR I* | - EcoR I* | G/AATTC | 37 ABH | 2,50 |
| 995 EcoR V | - EcoR V | GAT/ATC | 37 B | 10,00 |
| 2744 BstF5 I | neo Fok I | GGATG(2/0) | 65 A | 50,00 |
| 1056 Fok I | - Fok I | GGATG(9/13) | 37 A | 50,00 |
| 2743 BstH2 I | iso HaeII | RGCGC/Y | 65 A | 25,00 |
| 620 BsuR I | neo Hae III | GG/CC | 37 M | 5,00 |
| 1089 Hae III | - Hae III | GG/CC | 37 M | 10,00 |
| 15 AccB1 I | iso HgiC I | G/GYRCC | 37 extra | 25,00 |
| 2670 AspLE I | iso Hha I | GCG/C | 37 H | 25,00 |
| 2675 HspA I | neo Hha I | G/CGC | 37 A | 25,00 |
| 1151 Hind III | - Hind III | A/AGCTT | 37 BM | 2,50 |
| 1154 Hinf I(CG) | - Hinf I(CG) | G/ANTC | 37 ABH | 10,00 |
| 2862 BstHP I | iso Hpa I(CG) | GTT/AAC | 37 A | 50,00 |
| 1159 Hpa II(CG) | - Hpa II(CG) | C/CGG | 37 L | 25,00 |
| 1277 Msp I | iso Hpa II(CG) | C/CGG | 37 ABLM | 10,00 |
| 14 Acc65 Ic(CG) | neo Kpn I* | G/GTACC | 37 BH | 50,00 |
| 1180 Kpn I* | - Kpn I* | GGTAC/C | 37 L+BSA | 10,00 |
| 1186 Kzo9 I | iso Mbo Ia | /GATC | 37 M | 100,00 |
| 1213 Mfe I* | - Mfe I* | C/AATTG | 37 L | 100,00 |
| 1236 Mlu ICG | - Mlu ICG | A/CGCGT | 37 H | 25,00 |
| 1817 Tru9 I | iso Mse I | T/TAA | 65 ALM | 100,00 |
| 2676 MroN I | neo Nae I | G/CCGGC | 37 L | 25,00 |
| 2858 Ege I | neo Nar ICG | GGC/GCC | 37 L | 100,00 |
| 446 Bsp19 I | iso Nco I | C/CATGG | 37 BH | 25,00 |
| 1308 Nco I | - Nco I | C/CATGG | 37 H | 75,00 |
| 2736 FauND I | iso Nde I | CA/TATG | 37 A | 25,00 |
| 2854 AsuNH I | iso Nhe I(CG) | TT/CGAA | 37 L | 100,00 |
| 1367 Not ICG | - Not ICG | GC/GGCCGC | 37 H | 100,00 |
| 2673 CciN I | iso Not ICG | GC/GGCCGC | 37 A | 75,00 |
| 1371 Nru Ia CG | - Nru Ia CG | TCG/CGA | 37 B | 50,00 |
| 2156 Zsp2 I | iso Nsi I | ATGCA/T | 37 L | 50,00 |
| 4619 BstPA I* | iso PshA I | GACNN/NNGTC | 65 A | 25,00 |
| 1536 Pst I | - Pst I | CTGCA/G | 37 H | 5,00 |
| 1542 Pvu II | - Pvu II | CAG/CTG | 37 M | 10,00 |
| 1567 Rsa I | - Rsa I | CT/AC | 37 AL | 50,00 |
| 2679 Psp124B I | iso Sac I | GAGCT/C | 37 M | 25,00 |
| 1662 Sfr303 I | iso Sac IICG | CCGC/GG | 37 L | 25,00 |
| 1588 Sal ICG | - Sal ICG | G/TCGAC | 37 H | 10,00 |
| 2665 Acc113 I* | iso Sca I | AGT/ACT | 37 A | 25,00 |
| 1655 Sfi Ic(CG) | - Sfi Ic (CG) | GGCCNNNN/NGGCC | 50 M | 25,00 |
| 1704 Sma ICG | - Sma ICG | CCC/GGG | 25 A | 25,00 |
| 2138 Xma I | neo Sma ICG | C/CCGGG | 37 A | 250,00 |
| 2667 AclN I | iso Spe I | A/CTAGT | 150,00 | |
| 1719 Sph I | - Sph I | GCATG/C | 37 M | 150,00 |
| 2742 Sbf I | iso Sse8387 I | CCTGCA/GG | 37 A | 150,00 |
| 1746 Ssp I* | - Ssp I* | AAT/AAT | 37 H | 50,00 |
| 1496 Pme55 I | iso Stu Ic | AGG/GCT | 37 L | 25,00 |
| 554 BssT1 I | iso Sty I | C/CWWGG | 60 H | 25,00 |
| 2674 Erh I | iso Sty I | C/CWWGG | 37 H | 25,00 |
| 2750 Smi I | iso Swa I | ATTT/AAAT | 37 H | 100,00 |
| 1801 Taq Ia | - Taq Ia | T/CGA | 65 AB | 10,00 |
| 2639 Sse9 I | iso Tsp509 I | /AATT | 55 L | 150,00 |
| 2124 Vsp I | - Vsp I | AT/TAAT | 37 H | 25,00 |
| 2126 Xba Ia | - Xba Ia | T/CTAGA | 37 H | 10,00 |
| 1661 Sfr274 I | iso Xho ICG | C/TCGAG | 50 L | 20,00 |
| 2136 Xho ICG | - Xho ICG | C/TCGAG | 37 H | 10,00 |
| 2857 MroX I | iso Xmn I | GAANN/NNTTC | 37 H | 50,00 |
Copyright © 2002 M.B. Enzymes GmbH