Uracil DNA glycosylase.
Description	Recombinant E. coli uracil-DNA glycosylase (UDG, Deoxy-D-cyclobutadipyrimidine polynucleotido-deoxyribohydrolase, EC 3.2.2.17) catalyzes the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases) and RNA. This reaction leaves the DNA sugar-phosphodiester backbone intact. The resulting DNA is not suitable for use as hybridization target or as template for DNA synthesis.
Unit definition	One unit catalyzes the degradation of 1 µg ss uracil-containing DNA in 60 min at 37°C.
Reaction buffer	The enzyme works well in buffers used in PCR and RT protocols. Alternativelly, use 10x buffer consisted of 200 mM Tris-HCl pH 8.0, 10 mM DTT, 1 mg/ml BSA.
Reaction conditions	Treatment of 0,1 µg of uracil-containing DNA with 1 U of UDG for 10 min at 37°C renders the DNA incapable of being copied by DNA polymerase. The enzyme can be 95% irreversible inactivated by incubation at 95°C for 10 min.
Storage	Store at -20°C.
Applications	eliminates carryover contaminations that can result in false positives in PCR reactions; site-directed mutagenesis; cloning of PCR products.
Quality control	activity, SDS-PAGE purity, absence of exonuclease and unspecific endonuclease activities.
References	1. Lingahl, T. et al. (1977) J. Biol. Chem., 252, 3286-3294. 2. Longo, M. et al. (1990) Gene 93, 125. 3. Wang, Z. et al. (1991) Gene 99, 31-37. 4. Devchand, P.R. et al. (1993) Nucl. Acids Res. 21, 3437-3443.
Catalog #	M7.3	M7.4
Pack size	500 U	1000 U