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1 unit produces 1 µg of acid-soluble material per min at 37°C using 0.5 mg/ml heat denatured calf thymus DNA as substrate. |
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For 100 µl reaction use 10 U S1 nuclease, 2 µg DNA, 50 mM NaAc pH 4.6, 1 mM ZnAc, 250 mM NaCl, 50 mg/ml BSA. Incubate at 37°C for 30 min. Stop reaction by addition of 1 µl of 0.5 M EDTA. The reaction conditions will vary with different applications of S1 nuclease. | ||||
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Quality control |
activity, no detectable ds nuclease activity. |
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References |
1. Vogt, V.M. (1973) Eur. J. Biochem. 33, 192. 2. Roberts, T.M. et al. (1979) Proc. Natl. Acad. Sci. USA 76, 760-764. 3. Berk, A.J., Sharp, P.A. (1978) Proc. Natl. Acad. Sci. USA 75, 19274-1278. |
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Pack size |
10.000 U |
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Copyright © 2002 M.B. Enzymes GmbH