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One unit of the enzyme converts 10 nmol of dNTPs to an acid-insoluble form in 30 min at 37°C. |
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For fill-in reaction DNA should be present at conc. 50 mg/ml. Add 1 U Klenow, 1 mM dNTPs (end conc.), reaction buffer and incubate 30 min at 37°C. Excessive amount of the enzyme or prolonged incubation time may result in recessed ends due to the 3´-5´ exonuclease (proofreading) activity. |
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Quality control |
activity, absence of contaminating exo- und endonucleases, SDS-PAGE. |
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References |
1. Klenow, H. et al. (1971) Eur. J. Biochem. 22, 371-381. |
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L1.4 |
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Pack size |
500 U |
1000 U |
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Copyright © 2002 M.B. Enzymes GmbH